Label-free Isolation of Memory-type NK Cells for Cell Therapy R&D
Label-free Morphology-based Identification of T-Helper Cell Subsets
Assessment of Nuclear Morphology in Flow
Gentle Label-Free Sorting of iPSCs with Maintenance of Viability and Quality
Morphology-based Assessment of Viral Load in Mammalian Cells
Label-free Identification of Proliferative hMSCs
Classification of Mitochondrial Morphology in Activated T cells
Intracellular Protein Aggregation as a High Content Readout in Flow-Based Phenotypic Drug Discovery
TCY-AN-014.23.1-PDD-in-Flow (3)
Label-free assessment of B cell differentiation by Ghost Cytometry
Label Free Detection of Subtle Morphological Differences in Immune Cells
Classification of Cellular Diversity and Disease Assessment in ALL
Acute Lymphoblastic Leukemia (ALL) is a malignancy of lymphoid progenitor cells in the bone marrow and blood. The disease has a bimodal distribution with 80% of ALL patients presenting as children who respond positively to therapy. However, for adult patients ALL can be a devastating disease, with only 30–40% achieving long–term remission. Chromosomal abnormalities, such as the Philadelphia chromosome, are commonly found in ALL and are a major determinant of prognostic outcome. While long-term survival in pediatric ALL without chromosomal abnormalities approaches 90%, Philadelphia chromosome positive adult ALL has a five-year survival rate of 5 – 20%. As research into the genomic determinants of ALL disease progression and response to […]
Label-free Macrophage Subtyping
As monocytes migrate into tissues, they differentiate into macrophages. Upon activation, macrophages can polarize and adopt two very different and biologically opposing phenotypes, termed M1 and M2. M1 macrophages are considered classically activated macrophages that mediate proinflammatory responses while M2 macrophages mediate anti-inflammatory responses, both by cytokine secretion. The balance of M1 and/or M2 macrophage polarization is known to be a mediator of physiological responses in tumor progression and as such, the ability to isolate cells in the two polarization states in purified populations is crucial for understanding the dynamics of tumor progression. We show here that VisionSort can be used to isolate pure populations of M1 and M2 macrophages […]
Regulatory T cell Characterization and Isolation
T cell Activation
T cell activation is a critical step in the adaptive immune response. Studying the mechanisms of T cell activation is an important part of modern immunology research and in vitro activation is a requirement for cell therapy drug development programs. However, current methods to isolate activated T cells require labeling with T-cell markers (e.g. CD8) and key extracellular molecular activation indicators (such as CD38, CD45RO, and HLA-DR) with fluorescently tagged antibodies; a process that can interfere with downstream R&D assays and requires label removal in cell therapy development. The VisionSort platform enables label-free sorting of T cells, giving investigators and drug developers activated T cells, untouched by external labels, for […]
Label-Free High Throughput CRISPR-Based Screening for Morphological Phenotypes in Flow
The development of CRISPR-Cas9 genome editing technology has led to the emergence of a new generation of novel life sciences applications. In drug discovery, researchers have harnessed the precision of selective gene knockouts by CRISPR to enable genome-wide drug screening. By mapping genotypes to phenotypes, CRISPR-based phenotypic screens can enable a better understanding of drug mechanism of actions (MOAs) and identification of novel druggable targets. However, current phenotypic CRISPR screening approaches rely heavily on microscopic imaging of target phenotypes, a process that imposes throughput limitations and restricts screening to only a handful of simple phenotypes based on binary fluorescence signals. To fully realize the potential of CRISPRbased phenotypic screening, here […]
High Throughput CRISPR-Based Phenotypic Screening in Flow for Complex Intracellular Phenotypes
The development of CRISPR-Cas9 genome editing technology has led to the emergence of a new generation of novel life sciences applications1. In drug discovery, researchers have harnessed the precision of selective gene knockouts by CRISPR to enable genome-wide drug screening. By mapping genotypes to phenotypes, CRISPR-based phenotypic screens can enable a better understanding of drug mechanism of actions (MOAs) and identification of novel druggable targets. However, current phenotypic CRISPR screening approaches rely heavily on microscopic imaging of target phenotypes, a process that imposes throughput limitations and restricts screening to only a handful of simple phenotypes by binary fluorescence signals. To fully realize the potential of CRISPR-based phenotypic screening, here show […]
Intracellular Phenotyping with Enhanced Spatial Resolution in Flow
Identification and Isolation of CAR-T Cells with Enhanced Therapeutic Efficacy
Chimeric antigen receptor T-cells (CAR-T) have emerged as an exciting new approach for the treatment of cancer, with over 500 CAR-T worldwide clinical trials underway in 2021.1 Conventional thinking was that all CAR-T cells have similar therapeutic potential, however new research has found that not all CAR-T cells are created equal. A recent study showed that CAR-T cells with lower levels of glycolysis led to better clinical responses in leukemia patients.2 Here we use VisionSort to identify metabolically distinct CAR-T cell subsets without the use of molecular labels or tags as an approach to identify cells with the potential for higher therapeutic efficacy.
Label-free Assessment of Cell Viability
B cell Plasma Cell Differentiation
