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Regulatory T cells (Tregs) are specialized T cells that suppress immune responses by cytokine production and the inhibition of T cell proliferation. These specialized T cells are key for modulating the immune response and play a role in preventing autoimmunity. They also have a therapeutic role in preventing allograft rejection, allergies, and autoimmune diseases. Tregs have traditionally been defined by the expression of cell surface markers such as CD4 and CD25, and these labels are commonly used to identify these cells in flow cytometry applications.1 Cell therapy researchers need a supply of pure Treg cells, untouched by external labels, in order to perform functional studies and evaluate their utility as cellular therapies.2 Here we show here that VisionSort, powered by Ghost Cytometry technology, provides a solution to identify Treg subpopulations, without the use of labels, for downstream applications.

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