Label Free Detection of Subtle Morphological Differences in Immune Cells

Sensitive and high-throughput technologies to assess subtle morphological differences in cells are a major need for high-throughput drug screening and disease profiling research. However, conventional technologies like flow cytometry and microscopy to assess morphology have limitations. Microscopy is slow and a terminal assay for cells while flow cytometry only provides crude physical measures, like size and granularity, by detecting forward (FSC) and side (SSC) scattered light, respectively. By these measures, subtle morphological differences between cells cannot be detected. However, the data-rich profiles generated by Ghost Cytometry enable detection and resolution of ultra-fine differences in cell morphology. In this proof-of-concept study we use the VisionSort platform, powered by Ghost Cytometry, to […]

Classification of Cellular Diversity and Disease Assessment in ALL

Acute Lymphoblastic Leukemia (ALL) is a malignancy of lymphoid progenitor cells in the bone marrow and blood. The disease has a bimodal distribution with 80% of ALL patients presenting as children who respond positively to therapy. However, for adult patients ALL can be a devastating disease, with only 30–40% achieving long–term remission. Chromosomal abnormalities, such as the Philadelphia chromosome, are commonly found in ALL and are a major determinant of prognostic outcome. While long-term survival in pediatric ALL without chromosomal abnormalities approaches 90%, Philadelphia chromosome positive adult ALL has a five-year survival rate of 5 – 20%. As research into the genomic determinants of ALL disease progression and response to […]