Science
Science
Science

Platform

Cell Therapy

Label-free high throughput characterization and isolation of cell populations based on machine-predicted labels without the use of physical surface markers or stains.

Label-free
Monitoring and Isolation of Target Cells

Our Solution for Cell Therapy

Conventionally, surface markers or stains have been necessary to characterize and isolate target cells in the cell therapy manufacturing process. By applying VisionSort, we perform label-free characterization and isolation of cells at high throughput by using machine predicted “in-silico” labels. Our technology can classify and isolate cells based on morphological differences and has been used for identifying live and dead cells, activated and resting T cells, and differentiated and undifferentiated stem cells. It has also been used to assess more subtle morphological variations in cells and organelles including cell-cycle phases, glycolytic levels, and apoptotic status. This technology is highly compatible with automated manufacturing processes and since label-free cell characterization eliminates a sampling step, it enables inline measurements and enrichment in the cell manufacturing process.

Solution

Examples of cellular phenotypes distinguishable by VisionSort

  • Differentiated

    Differentiated

    Undifferentiated

    Undifferentiated

    Activated

    Activated

    Resting

    Resting

  • Lymphocyte

    Lymphocyte

    Monocyte

    Monocyte

    Low Glycolysis

    Low Glycolysis

    High Glycolysis

    High Glycolysis

  • Live

    Live

    Apoptotic

    Apoptotic

    Cancerous

    Cancerous

    Healthy

    Healthy

* Images acquired from the FlowSight system (Luminex)

Key Advantages of Our Technology

Label-free process

Label-free process

Cells can be characterized and isolated without the use of molecular labels, ideal for automated cell manufacturing

Closed system

Closed system

Cell monitoring and isolation processes are performed within a sterile and closed system

High throughput

High throughput

Image-based cell sorting at a high throughput rate, over 3,000 cells per second

New imaging “biomarkers”

New imaging “biomarkers”

Image-based profiling and isolation of cells lead to new biological findings