Platform
Cell Therapy
Label-free high throughput characterization and isolation of cell populations based on machine-predicted labels without the use of physical surface markers or stains.
Label-free
Monitoring and Isolation of Target
Cells
Our Solution for Cell Therapy
Conventionally, surface markers or stains have been necessary to characterize and isolate target cells in the cell therapy manufacturing process. By applying Ghost Cytometry, we perform label-free characterization and isolation of cells at high throughput by using machine predicted “in-silico” labels. Our technology can classify and isolate cells based on morphological differences and has been used for identifying live and dead cells, activated and resting T cells, and differentiated and undifferentiated stem cells. It has also been used to assess more subtle morphological variations in cells and organelles including cell-cycle phases, glycolytic levels, and apoptotic status. This technology is highly compatible with automated manufacturing processes and since label-free cell characterization eliminates a sampling step, it enables inline measurements and enrichment in the cell manufacturing process.
Examples of cellular phenotypes distinguishable by Ghost Cytometry
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Differentiated
Undifferentiated
Activated
Resting
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Lymphocyte
Monocyte
Low Glycolysis
High Glycolysis
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Live
Apoptotic
Cancerous
Healthy
* Images acquired from the FlowSight system (Luminex)
Key Advantages of Our Technology
Label-free process
Cells can be characterized and isolated without the use of molecular labels, ideal for automated cell manufacturing
Closed system
Cell monitoring and isolation processes are performed within a sterile and closed system
High throughput
Image-based cell sorting at a high throughput rate, over 3,000 cells per second
New imaging “biomarkers”
Image-based profiling and isolation of cells lead to new biological findings